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Registro completo
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Biblioteca (s) : |
INIA La Estanzuela. |
Fecha : |
29/10/2020 |
Actualizado : |
27/01/2021 |
Tipo de producción científica : |
Artículos en Revistas Indexadas Internacionales |
Autor : |
GIANNITTI, F.; GARCÍA, J.P.; ROOD, J.I.; ADAMS, V.; ARMENDANO, J.I.; BEINGESSER, J.; UZAL, F.A. |
Afiliación : |
FEDERICO GIANNITTI, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; JORGE P. GARCÍA, Universidad Nacional del Centro de la Provincia de Buenos Aires (UNCPBA), Tandil, Buenos Aires, Argentina.; JULIAN I. ROOD, Monash University, Clayton, Victoria, Australia.; VICKI ADAMS, Monash University, Clayton, Victoria, Australia; JOAQUÍN I. ARMENDANO, Universidad Nacional del Centro de la Provincia de Buenos Aires (UNCPBA), Tandil, Buenos Aires, Argentina.; JULIANN BEINGESSER, University of California at Davis, San Bernardino, CA, USA.; FRANCISCO A. UZAL, University of California at Davis, San Bernardino, CA, USA. |
Título : |
Cardiopulmonary Lesions in Sheep Produced by Experimental Acute Clostridium Perfringens Type D Enterotoxemia. |
Fecha de publicación : |
2021 |
Fuente / Imprenta : |
Veterinay Pathology, volumen 58, number 1, pag. 103-113, 2021. Doi: https://doi.org/10.1177/0300985820965554 |
DOI : |
10.1177/0300985820965554 |
Idioma : |
Inglés |
Notas : |
Article history: First Published October 15, 2020. Corresponding Author:Francisco A. Uzal, Email: fuzal@cahfs.ucdavis.edu. The author(s) disclosed receipt of the following financial support for the research, authorship, and/or publication of this article: Federico Giannitti acknowledges support from the Uruguayan ?Agencia Nacional de Investigaci ´on e Innovaci ´on? (ANII) through mobility grant MOV_CA_2018_1_150021. This work was supported by Grant R01 AI056177 from the National Institute of Allergy and Infectious Diseases (NIAID). Research at Monash University was also supported by funding provided by the Australian Research Council to the Australian Research Council Centre of Excellence in Structural and Functional Microbial Genomics (Grant CE0562063). |
Contenido : |
Abstract:Enterotoxemia caused by Clostridium perfringens type D is one of the most prevalent clostridial diseases of sheep. The lesions of
the acute form of this disease, particularly the cerebral lesions, are well characterized; however, detailed descriptions of the cardiac and pulmonary lesions are lacking. Here we describe cardiopulmonary lesions in experimental acute type D enterotoxemia in sheep and determine the role of epsilon toxin (ETX) in the development of these lesions. Four groups of 6 sheep were intraduodenally inoculated with either a wild-type C. perfringens type D strain; its etx knockout mutant, which is unable to produce ETX; the etx mutant complemented with the wild-type etx gene, which regains the ETX toxigenic ability; or sterile culture
medium as a control. All sheep were subjected to postmortem examination within 24 hours of inoculation. Lesion scores were compared between groups for pulmonary edema; hydrothorax; ascites; hydropericardium; endocardial, myocardial and epicardial hemorrhages; microscopic lesions of acute myocardial degeneration and necrosis; and myocardial, endocardial, and epicardial edema, hemorrhage, and inflammation. Only sheep inoculated with the wild-type and complemented ETX-toxigenic bacterial strains developed cardiopulmonary lesions, which were present in varying degrees of severity and proportions. These lesions were not present in sheep inoculated with the etx mutant or in the negative control. We conclude that severe acute cardiopulmonary lesions in sheep with experimental enterotoxemia are associated with the capacity of the strains to produce ETX. These changes are likely contributors to the clinical signs and even death of affected animals. MenosAbstract:Enterotoxemia caused by Clostridium perfringens type D is one of the most prevalent clostridial diseases of sheep. The lesions of
the acute form of this disease, particularly the cerebral lesions, are well characterized; however, detailed descriptions of the cardiac and pulmonary lesions are lacking. Here we describe cardiopulmonary lesions in experimental acute type D enterotoxemia in sheep and determine the role of epsilon toxin (ETX) in the development of these lesions. Four groups of 6 sheep were intraduodenally inoculated with either a wild-type C. perfringens type D strain; its etx knockout mutant, which is unable to produce ETX; the etx mutant complemented with the wild-type etx gene, which regains the ETX toxigenic ability; or sterile culture
medium as a control. All sheep were subjected to postmortem examination within 24 hours of inoculation. Lesion scores were compared between groups for pulmonary edema; hydrothorax; ascites; hydropericardium; endocardial, myocardial and epicardial hemorrhages; microscopic lesions of acute myocardial degeneration and necrosis; and myocardial, endocardial, and epicardial edema, hemorrhage, and inflammation. Only sheep inoculated with the wild-type and complemented ETX-toxigenic bacterial strains developed cardiopulmonary lesions, which were present in varying degrees of severity and proportions. These lesions were not present in sheep inoculated with the etx mutant or in the negative control. We conclude that severe acute ... Presentar Todo |
Palabras claves : |
CLOSTRIDIUM PERFRINGENS TYPE D; EPSILON TOXIN; HEART; LUNGS; PATHOLOGY; PLATAFORMA SALUD ANIMAL; SHEEP. |
Thesagro : |
ENFERMEDADES DE LOS ANIMALES; OVEJA. |
Asunto categoría : |
-- |
Marc : |
LEADER 03466naa a2200325 a 4500 001 1061439 005 2021-01-27 008 2021 bl uuuu u00u1 u #d 024 7 $a10.1177/0300985820965554$2DOI 100 1 $aGIANNITTI, F. 245 $aCardiopulmonary Lesions in Sheep Produced by Experimental Acute Clostridium Perfringens Type D Enterotoxemia.$h[electronic resource] 260 $c2021 500 $aArticle history: First Published October 15, 2020. Corresponding Author:Francisco A. Uzal, Email: fuzal@cahfs.ucdavis.edu. The author(s) disclosed receipt of the following financial support for the research, authorship, and/or publication of this article: Federico Giannitti acknowledges support from the Uruguayan ?Agencia Nacional de Investigaci ´on e Innovaci ´on? (ANII) through mobility grant MOV_CA_2018_1_150021. This work was supported by Grant R01 AI056177 from the National Institute of Allergy and Infectious Diseases (NIAID). Research at Monash University was also supported by funding provided by the Australian Research Council to the Australian Research Council Centre of Excellence in Structural and Functional Microbial Genomics (Grant CE0562063). 520 $aAbstract:Enterotoxemia caused by Clostridium perfringens type D is one of the most prevalent clostridial diseases of sheep. The lesions of the acute form of this disease, particularly the cerebral lesions, are well characterized; however, detailed descriptions of the cardiac and pulmonary lesions are lacking. Here we describe cardiopulmonary lesions in experimental acute type D enterotoxemia in sheep and determine the role of epsilon toxin (ETX) in the development of these lesions. Four groups of 6 sheep were intraduodenally inoculated with either a wild-type C. perfringens type D strain; its etx knockout mutant, which is unable to produce ETX; the etx mutant complemented with the wild-type etx gene, which regains the ETX toxigenic ability; or sterile culture medium as a control. All sheep were subjected to postmortem examination within 24 hours of inoculation. Lesion scores were compared between groups for pulmonary edema; hydrothorax; ascites; hydropericardium; endocardial, myocardial and epicardial hemorrhages; microscopic lesions of acute myocardial degeneration and necrosis; and myocardial, endocardial, and epicardial edema, hemorrhage, and inflammation. Only sheep inoculated with the wild-type and complemented ETX-toxigenic bacterial strains developed cardiopulmonary lesions, which were present in varying degrees of severity and proportions. These lesions were not present in sheep inoculated with the etx mutant or in the negative control. We conclude that severe acute cardiopulmonary lesions in sheep with experimental enterotoxemia are associated with the capacity of the strains to produce ETX. These changes are likely contributors to the clinical signs and even death of affected animals. 650 $aENFERMEDADES DE LOS ANIMALES 650 $aOVEJA 653 $aCLOSTRIDIUM PERFRINGENS TYPE D 653 $aEPSILON TOXIN 653 $aHEART 653 $aLUNGS 653 $aPATHOLOGY 653 $aPLATAFORMA SALUD ANIMAL 653 $aSHEEP 700 1 $aGARCÍA, J.P. 700 1 $aROOD, J.I. 700 1 $aADAMS, V. 700 1 $aARMENDANO, J.I. 700 1 $aBEINGESSER, J. 700 1 $aUZAL, F.A. 773 $tVeterinay Pathology, volumen 58, number 1, pag. 103-113, 2021. Doi: https://doi.org/10.1177/0300985820965554
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INIA La Estanzuela (LE) |
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Biblioteca (s) : |
INIA La Estanzuela. |
Fecha actual : |
10/07/2017 |
Actualizado : |
17/07/2017 |
Tipo de producción científica : |
Artículos en Revistas Indexadas Nacionales |
Circulación / Nivel : |
Nacional - -- |
Autor : |
BERETTA, A.; BASSAHUN, D.; TORRES, D.; MUSSELLI, R.; GARCIA, L. |
Afiliación : |
ANDRES NICOLAS BERETTA BLANCO, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; DANIEL FERNANDO BASSAHUN RODRIGUEZ, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; DEBORAH LUCIANA TORRES GUERRERO, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; BEATRIZ RAQUEL MUSSELLI NEGRIN, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; LETICIA IRENE GARCIA BARRETO, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay. |
Título : |
Acidez titulable a pH = 7 estimada a partir del pH de una mezcla suelo:buffer. (Titratable Acidity at pH = 7 Estimated from the pH of a Soil:Buffer Mixture). |
Fecha de publicación : |
2017 |
Fuente / Imprenta : |
Agrociencia Uruguay, v. 21, n.1, p. 105-108, 2017. |
Idioma : |
Español |
Notas : |
Article history: Recibido: 2015-11-19 Aceptado: 2016-11-23 |
Contenido : |
Resumen:
El valor de acidez titulable a pH = 7 en suelo (AT_pH7) se utiliza con fines taxonómicos y para estimar la necesidad de
encalado. Es posible predecir AT_pH7 por el valor de pH de la mezcla suelo:solución buffer (pH_equilibrio). A cada muestra
se le agregó acetato de calcio buffereado a pH = 7, se midió el pH_equilibrio y el álcali necesario para llegar a pH=7. Con
pH_equilibrio se estimó AT_pH7 por regresión (AT_eq) o por la cantidad de álcali neutralizado para alcanzar el pH_equilibrio
(AT_OH). Existió un ajuste aceptable entre AT_pH7, AT_eq y AT_OH, pero sus diferencias no tuvieron distribución normal.
Con AT_OH y AT_eq, se subestimó el promedio de AT_pH7. El valor de pH_equilibrio fue más preciso que AT_pH7, en tanto
que las medidas AT_eq y AT_OH tuvieron menor precisión. Al utilizar AT_eq y AT_OH, no se introdujeron errores significativos
al clasificar los suelos por su saturación en bases. Ambas mediciones se realizaron en menor tiempo que AT_pH7. |
Palabras claves : |
ACIDEZ POTENCIAL; BASE SATURATION; CAPACIDAD INTERCAMBIO; CATION EXCHANGE CAPACITY; CATIÓNICO; DESATURATED SOIL; POTENTIAL ACIDITY; SATURACIÓN EN BASES; SATURATED SOIL; SUELO DESATURADO; SUELO SATURADO. |
Asunto categoría : |
P30 Ciencia del suelo y manejo del suelo |
URL : |
http://www.ainfo.inia.uy/digital/bitstream/item/7064/1/Agrociencia-21-1p.105-108.pdf
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Marc : |
LEADER 02000naa a2200313 a 4500 001 1057361 005 2017-07-17 008 2017 bl uuuu u00u1 u #d 100 1 $aBERETTA, A. 245 $aAcidez titulable a pH = 7 estimada a partir del pH de una mezcla suelo$bbuffer. (Titratable Acidity at pH = 7 Estimated from the pH of a Soil:Buffer Mixture).$h[electronic resource] 260 $c2017 500 $aArticle history: Recibido: 2015-11-19 Aceptado: 2016-11-23 520 $aResumen: El valor de acidez titulable a pH = 7 en suelo (AT_pH7) se utiliza con fines taxonómicos y para estimar la necesidad de encalado. Es posible predecir AT_pH7 por el valor de pH de la mezcla suelo:solución buffer (pH_equilibrio). A cada muestra se le agregó acetato de calcio buffereado a pH = 7, se midió el pH_equilibrio y el álcali necesario para llegar a pH=7. Con pH_equilibrio se estimó AT_pH7 por regresión (AT_eq) o por la cantidad de álcali neutralizado para alcanzar el pH_equilibrio (AT_OH). Existió un ajuste aceptable entre AT_pH7, AT_eq y AT_OH, pero sus diferencias no tuvieron distribución normal. Con AT_OH y AT_eq, se subestimó el promedio de AT_pH7. El valor de pH_equilibrio fue más preciso que AT_pH7, en tanto que las medidas AT_eq y AT_OH tuvieron menor precisión. Al utilizar AT_eq y AT_OH, no se introdujeron errores significativos al clasificar los suelos por su saturación en bases. Ambas mediciones se realizaron en menor tiempo que AT_pH7. 653 $aACIDEZ POTENCIAL 653 $aBASE SATURATION 653 $aCAPACIDAD INTERCAMBIO 653 $aCATION EXCHANGE CAPACITY 653 $aCATIÓNICO 653 $aDESATURATED SOIL 653 $aPOTENTIAL ACIDITY 653 $aSATURACIÓN EN BASES 653 $aSATURATED SOIL 653 $aSUELO DESATURADO 653 $aSUELO SATURADO 700 1 $aBASSAHUN, D. 700 1 $aTORRES, D. 700 1 $aMUSSELLI, R. 700 1 $aGARCIA, L. 773 $tAgrociencia Uruguay$gv. 21, n.1, p. 105-108, 2017.
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